Acinetobacter nematophilus sp. nov., Alcaligenes nematophilus sp. nov., Enterobacter nematophilus sp. nov., and Kaistia nematophila sp. nov., Isolated from Soil-Borne Nematodes and Proposal for the Elevation of Alcaligenes faecalis subsp. faecalis, Alcaligenes faecalis subsp. parafaecalis, and Alcaligenes faecalis subsp. phenolicus to the Species Level

Four bacterial strains, A-IN1T, A-TC2T, E-TC7T, and K-TC2T, isolated from soil-borne nematodes of the species Oscheius tipulae Acrobeloides bodenheimeri, were found to represent new genera Acinetobacter, Alcaligenes, Enterobacter, Kaistia, respectively. In this study, we described these using a polyphasic taxonomic approach that included whole-genome whole-proteome phylogenomic reconstructions, core genome sequence comparisons, phenotypic characterization. Phylogenomic reconstructions sequences show A-IN1T is closely related Acinetobacter guillouiae DSM 590T bereziniae LMG 1003T. The dDDH values between latest strains are 25.1 39.6%, respectively, which below 70% divergence threshold for prokaryotic delineation. A-TC2T Alcaligenes faecalis subsp. 30030T phenolicus 16503T. 47.0 66.3%, addition, 30030T, 16503T, parafaecalis always lower than 70%, demonstrating three within genus rather subspecies faecalis. E-TC7T Enterobacter kobei 13645T, chuandaensis 090028T, bugandensis STN0717-56T. 43.5, 42.9, 63.7%, K-TC2T Kaistia terrae 21341T defluvii JCM 18034T. 29.2 30.7%, Several biochemical tests allow differentiate type newly their more species. Based on results approach, following proposed: nematophilus sp. nov. with (=CCM 9231T =CCOS 2018T) as strain, 9230T 2017T) 9232T 2020T) nematophila 9239T 2022T) strain. propose elevation faecalis, parafaecalis, level. Therefore, creation 13975T 16503T Our study contributes better understanding biodiversity phylogenetic relationships bacteria associated nematodes.